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KMID : 0378019920350010054
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New Medical Journal
1992 Volume.35 No. 1 p.54 ~ p.60
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Effect of Bradykinin and Indomethacin on the Active Transport of Sodium in IMCD Cells of Rats
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Abstract
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The renal kallikrein-kinin system has been postulated to play a role in regulating renal hemodynamics and electrolyte excretion. Many studies in intact animals have indicated that bradykinin, one of the effective peptides of the kallikrein-kinin system, is natriuretic. However, it is not clear whether natriuresis results from the renal hemodynamic effects or from the direct effect on renal tubular sodium transport. Therefore, to elucidate the role of bradykinin in sodium handling of IMCD (inner medullary collecting duct), we examined the effect of bradykinin on the transport-dependent oxygen consumption in IMCD cells of rats. The relationship between the effect of bradykinin on oxygen consumption and prostaglandin was also investigated. Bradykinin inhibited oxygen consumption in a dose-dependent way with a maximal reduction at 10-´ M bradykinin. The inhibitory effect of bradykinin was not present in the absence of sodium or in the presence of ouabain (10-° M). These data imply that the inhibitory effect of bradykinin is restricted to the sodium transport-dependent oxygen consumption. Indomethacin (0.5 mM) prevented the inhibitory effect of bradykinin. These results suggest´ that bradykinin decreases the active sodium transport in IMCD, and that it may be mediated by prostaglandin.
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